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Original article
- Effect of in vitro testicular spermatozoa culture on pregnancy outcomes: an experience at a single university hospital
- Jisun Lee, Jung Hyeon Yoo, Jae Hun Lee, Hyun Soo Ahn, Kyung Joo Hwang, Miran Kim
- Yeungnam Univ J Med. 2021;38(1):53-59. Published online December 9, 2020
- DOI: https://doi.org/10.12701/yujm.2020.00773
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- 2 Crossref
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Abstract
PDF - Background
There are no guidelines for the optimal incubation time or temperature to improve pregnancy outcomes in testicular sperm extraction-intracytoplasmic sperm injection (TESE-ICSI) cycles. We aimed to evaluate whether a 24-hour in vitro culture of testicular spermatozoa affects pregnancy outcomes in TESE-ICSI cycles.
Methods
This was a retrospective study of 83 TESE-ICSI cycles using testicular spermatozoa in 46 couples with male partners suffering from nonobstructive or obstructive azoospermia. Sperm retrieval was performed either on the oocyte retrieval (OR) day (65 cycles in 33 couples; group A) or on the day before OR (18 cycles in 13 couples; group B) followed by in vitro culture for 24 hours. The clinical characteristics and pregnancy outcomes, including the number of retrieved oocytes, fertilization rates, embryo transfer rates, implantation and clinical pregnancy rates, were compared between the two groups.
Results
There were no differences in terms of clinical characteristics except for the levels of luteinizing hormone (LH) in males. Group B had higher LH levels than group A (4.56±1.24 IU/L vs. 3.67±1.07 IU/L, p=0.017). Group B showed higher fertilization rate (72.4±32.1% vs. 59.2±21.7%, p=0.045), implantation rate (35.0±34.1% vs. 14.0±21.5%, p=010), pregnancy rate per cycle (80% vs. 39%, p=0.033), and clinical pregnancy rate per cycle (80% vs. 37.5%, p=0.024) than those of group A.
Conclusion
Testicular sperm retrieval performed on the day before OR followed by in vitro culture can potentially improve pregnancy outcomes. -
Citations
Citations to this article as recorded by
- Intelligent Medicine on the Regulation Effect of Weekend Catch-Up Sleep on the Relationship Between Hypertension and All-Cause Mortality
Hui Li, Min Yang, Yanchun Bao
International Journal of Computational Intelligence Systems.2023;[Epub] CrossRef - Extend the Survival of Human Sperm In Vitro in Non-Freezing Conditions: Damage Mechanisms, Preservation Technologies, and Clinical Applications
Qingyuan Cheng, Liman Li, Min Jiang, Bo Liu, Yang Xian, Shasha Liu, Xiao Liu, Wenrui Zhao, Fuping Li
Cells.2022; 11(18): 2845. CrossRef
- Intelligent Medicine on the Regulation Effect of Weekend Catch-Up Sleep on the Relationship Between Hypertension and All-Cause Mortality
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