Journal of Yeungnam Medical Science

Original article

Microbiology
Adenylate kinase gene polymerase chain reaction-restriction fragment length polymorphism to differentiate Escherichia coli, Escherichia fergusonii, and Citrobacter freundii: a molecular diagnostic study: Ram Hari Dahal, Yoon-Jung Choi, Bokyung Kim, Md Shohel Rana, Joo Hun Shin, Jungmin Kim, Shukho Kim; J Yeungnam Med Sci. 2025;42:52. Published online September 1, 2025; DOI: https://doi.org/10.12701/jyms.2025.42.52

1,044 View
69 Download

Abstract PDF Supplementary Material: Background
Escherichia coli (EC), Escherichia fergusonii (EF), and Citrobacter freundii (CF) are clinically significant Gram-negative bacteria that are difficult to differentiate because of their shared 16S rRNA gene sequences.
Methods
This study presents a novel approach utilizing adenylate kinase (adk) gene polymerase chain reaction (PCR)-restriction fragment length polymorphism analysis for the precise discrimination of EC, EF, and CF. Analysis of adk sequences revealed unique restriction sites for BtsIMutI, BtgI, and AgeI restriction enzymes at nucleotide positions 93 and 96.
Results
Theoretical predictions translated into distinct banding patterns during agarose gel electrophoresis following PCR/restriction digestion. Experimental validation with reference strains and clinical isolates, including 84 EC and CF strains, demonstrated the efficacy of this method for differentiating these species. Assay specificity was confirmed by selective digestion of adk amplicons with BtsIMutI for EC and AgeI for CF.
Conclusion
This molecular technique provides a rapid and accurate method to discriminate between closely related bacterial species and is promising for clinical diagnostics and epidemiological studies. Thus, our adk PCR/restriction digestion assay is a valuable tool for the advancement of bacterial typing methods for EC, EF, and CF, and contributes to the ongoing exploration of microbial diversity and epidemiology.

Review

Anatomy
Adult Mesenchymal Stem Cells for Cell Therapy in Clinical Application.: In Hwan Song; Yeungnam Univ J Med. 2009;26(1):1-14. Published online June 30, 2009; DOI: https://doi.org/10.12701/yujm.2009.26.1.1

3,095 View
11 Download
3 Crossref

Human bone marrow-derived mesenchymal stem cells (MSCs) are a rare population of undifferentiated cells that have the capacity of self renewal and the ability to differentiate into mesodermal phenotypes, including osteocytes, chondrocytes, and adipocytes in vitro. Recently, MSCs have been shown to reside within the connective tissue of most organs, and their surface phenotype has been well analyzed. Many reports showed that transplanted MSCs enhanced regeneration as well as functional improvement of damaged organs and tissues. The wide differentiation plasticity of MSCs was expected to contribute to their demonstrated efficacy in a wide variety of experimental animal models and in human clinical trials. However, new findings suggest that the ability of MSCs to alter the tissue microenvironment via secretion of soluble factors may contribute more significantly than their capacity for differentiation in tissue repair. This review describes what is known about the cellular characteristics and differentiation potential of MSCs, which represent a promising stem cell population for further applications in regenerative medicine.

Citations

Citations to this article as recorded by

Application of Bio-Active Elastin-like Polypeptide on Regulation of Human Mesenchymal Stem Cell Behavior
Vijaya Sarangthem, Harshita Sharma, Mohini Mendiratta, Ranjit Kumar Sahoo, Rang-Woon Park, Lalit Kumar, Thoudam Debraj Singh, Sujata Mohanty
Biomedicines.2022; 10(5): 1151. CrossRef
Mesenchymal Stem Cells Decrease Oxidative Stress in the Bowels of Interleukin-10 Knockout Mice
Kyong Jin Jung, Gun Woo Lee, Chul Hyun Park, Tae Jin Lee, Joo Young Kim, Eon Gi Sung, Seong Yong Kim, Byung Ik Jang, In Hwan Song
Gut and Liver.2020; 14(1): 100. CrossRef
Human adipose-derived stem cells attenuate inflammatory bowel disease in IL-10 knockout mice
Woo Yeun Jung, Joo Hwan Kang, Kyung Gon Kim, Hee Snn Kim, Byung Ik Jang, Yong Hoon Park, In-Hwan Song
Tissue and Cell.2015; 47(1): 86. CrossRef

Original Article

Gastroenterology and Hepatology
Ascitic Fluid Analysis for the Differentiation of Malignancy-Related and Nonmalignant Ascites.: Eun Young Lee, Byeoung Deok Kim, Jae Hyuk Choi, Sang Yeop Lee, Hun Mo Ryu, Kyung Hee Lee, Myung Soo Hyun; Yeungnam Univ J Med. 1999;16(1):76-84. Published online June 30, 1999; DOI: https://doi.org/10.12701/yujm.1999.16.1.76

2,272 View
3 Download

Abstract PDF: The differentiation between Malignancy-Related Ascites(MRA) and Non-Malignant Ascites (NMA) is important for further diagnostic and therapeutic procedures. Althought many parameters were investigated, none has provided a complete distinction between MRA and NMA. We investigated several ascitic fluid parameters to determine the differential power, and to ifferentiate malignant-related from nonmalignant-related ascites with a sequence of sensitive parameters followed by specific parameters. For the present sturdy, 80 patients with ascites were divided into two groups: MRA and NMA. The MRA group was consisted of 27 patients with proven malignancy by image study, biopsy, and follow up; 21 of these patients had peritoneal carcinomatosis, but the remaining 6 showed no evidence of peritoneal carcinomatosis. The NMA group was consisted of 53 patients with no evidence of malignancy; among these patients, one had SLE, and others had liver cirrhosis. The samples of blood and ascites were obtained simultaneously, and then the levels of ascites cholesterol, CEA, protein, LDH, cytology, albumin gradient, ascites/serum concentration ratios of LDH(LDH A/S), and ascites/serum concentration ratios of protein(protein A/S) were measured. Applying cut-off limits for determined parameters, we estimated the diagnostic efficacy of each parameter. Among the eight parameters investigated, ascites fluid cholesterol yielded the best sensitive value of 93%(cut-off value 30mg/dl), and cytologic examination and the protein A/S(cut-off value 0.5) showed the most specific value of 100% and 96%, respectively. Based on the above result, the diagnostic sequence with cholesterol as a sensitive parameter, followed by the combination of cytologic examination and protein A/S as specific parameters, was tested in 80 patients. This diagnostic sequence identified 81.5% of patients with malignancy, and all patients with peritoneal carcinomatosis were classified as malignancy-related ascites. In spite of many limitations, this proposed diagnostic sequence may permit a cost-effective and simple differentiation of malignacy-related ascites from nonmalignant ascites

First
Prev
Page of 1
Next
Last

Search