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HOME > J Yeungnam Med Sci > Volume 24(2 Suppl); 2007 > Article
Original Article Differential Expression of Chemokines in Vascular Smooth Muscle Cells from Spontaneously Hypertensive Rat and Normotensive Rat
Jung Hae Kim1, So Young Park2, Hee Sun Kim1
Journal of Yeungnam Medical Science 2007;24(2 Suppl):S373-383
DOI: https://doi.org/10.12701/yujm.2007.24.2S.S373
Published online: December 31, 2007
1Department of Microbiology College of Medicine, Yeungnam University, Daegu, Korea
2Department of Physiology College of Medicine, Yeungnam University, Daegu, Korea
Corresponding author:  Hee Sun Kim, Tel: (053) 620-4363, Fax: (053) 653-6628, 
Email: heesun@med.yu.ac.kr
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Background
:The action of chemokines to the vascular inflammation plays a pathogenic role in the development and maintenance of hypertension. Materials and methods:In the present study, the expression of chemokine IL-8/CXCL8, MCP-1/CCL2 and RANTES/CCL5 was investigated in cultured vascular smooth muscle cells (VSMC) obtained from the thoracic aorta of normotensive Wister-Kyoto rat (WKY) and spontaneously hypertensive rat (SHR). We used real-time PCR and western blotting.
Result
:The expressions of IL-8/CXCL8, and MCP-1/CCL2 mRNA were stronger in VSMC from SHR than in WKY. However, the expression of RANTES/CCL5 was stronger in VSMC from WKY than in SHR. Expressions of CXCR1, CCR2, CD14 and PPARγ mRNA were stronger in VSMC from WKY than in SHR. Expressions of LPS-induced IL-8/CXCL8 and MCP-1/CCL2 mRNA were stronger in VSMC from SHR, but expression of LPS-induced RANTES/CCL5 was stronger in VSMC from WKY. A PPAR-γ ligand, 15-deoxy-Δ12, 14- prostaglandin J2 (15d-PGJ2) which possesses anti-inflammatory activity suppressed the expressions of LPS-induced IL-8/CXCL8, MCP-1/CCL2 and RANTES/CCL5 in VSMC from WKY and the expressions of LPS-induced MCP-1/CCL2 and RANTES/CCL5 expressions in SHR. But, the expression of LPS-induced IL-8/CXCL8 mRNA in SHR was increased by 15d- PGJ2. Angiotensin II (AngII) also induced IL-8/CXCL8 and MCP-1/CCL2 mRNA expressions in VSMC from SHR, but inhibited the expression of RANTES/CCL5 mRNA. Activities of LPS, or AngII-induced MAP kinases were stronger in VSMC from SHR than in WKY. Expression of AngII-induced IL-8/CXCL8 mRNA was associated with ERK phathway, and the expression of AngII-induced MCP-1/CCL2 mRNA was associated with p38 pathway, and the inhibition of RANTES/CCL5 mRNA by AngII was not associated with MAP Kinases pathways.
Conclusion
:Chemokine IL-8/CXCL8 and MCP-1/CCL2, not RANTES/CCL5, has a possibility to play a critical role in the pathogenesis of hypertension in the SHR.

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